aqueous extract :水溶性萃取
MCF7 cells: 乳癌細胞的名字,實驗室裡做實驗用的
根據wiki的解釋 : Overexpression of c-jun in MCF-7 cells can result in overall increased aggressiveness(細節請自己點進去看)
2. Brefeldin A is an estrogenic, Erk1/2-activating component in the extract of Agaricusblazei mycelia.
巴西蘑菇對於很多癌症是有幫助的 : http://www.ncbi.nlm.nih.gov/pubmed/?term=Agaricus+blazei+%2C+cancer
註: 原致癌基因（Proto-oncogene）: http://highscope.ch.ntu.edu.tw/wordpress/?p=42585
We first examined the effect of the A. blazei extract on cell growth using breast cancer MCF-7 cells and found that the extract inhibited cell growth in a dose-dependent manner, 50% at 4 μg/ml and 80% at 6 and 8 μg/ml (data not shown). This finding was not surprising because such inhibitory activity had been reported (Grube et al. 2001). We assumed that any potential estrogen-like activity would be masked by this growth-suppressive effect. We therefore examined whether the A. blazei extract shows activity similar to estrogen at the gene expression level using a customized DNA microarray containing a set of 150 estrogen-responsive genes (Fig. 1A). Similar expression profiles were obtained between cells treated with 10 nM E and those treated with 2 μg/ml of the A. blazei extract (correlation coefficient or R = 0.84), suggesting the presence of estrogen-like activity in the extract. The 150 genes were further categorized into six groups with specific functions (enzymes, signaling, proliferation, transcription, transport and others) and used for a correlation analysis (Fig. 1B). Significant correlations were observed between the A. blazei extract and E for the groups enzymes, signaling, transcription, transport and others, while a significant but less correlation was observed for the group proliferation (Fig. 1B).